Journal: International Journal of Molecular Sciences

Article Title: A Novel Triplet-Primed PCR Assay to Detect the Full Range of Trinucleotide CAG Repeats in the Huntingtin Gene ( HTT )

doi: 10.3390/ijms22041689

Figure Lengend Snippet: Huntington disease (HD) reference samples with published allele sizes [ 30 ] compared to those determined using the AmplideX PCR/CE HTT Kit.

Article Snippet: The AmplideX PCR/CE HTT Kit (cat#: 49657; Asuragen, Inc., Austin, TX, USA) was used to PCR amplify the HTT trinucleotide CAG fragment starting from 20 ng total of purified genomic DNA, isolated from peripheral blood leukocytes using the Gentra Puregene Blood Kit (Qiagen, Germantown, MD, USA), or the MagCore Genomic DNA Whole Blood Kit (Diatech Lab line, Jesi, Italy).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Novel Triplet-Primed PCR Assay to Detect the Full Range of Trinucleotide CAG Repeats in the Huntingtin Gene ( HTT )

doi: 10.3390/ijms22041689

Figure Lengend Snippet: Known polymorphisms adjacent to or within the CAG repeat tract. The AmplideX PCR/CE HTT Kit accurately genotypes templates with known polymorphisms adjacent to or within the CAG repeat tract. ( A ) Template with 19 repeats showing the expected CE electropherogram and corresponding genotype for the relevant portion of the HTT reference sequence. ( B ) DNA with two SNPs (rs473915 A>G and rs76533208 A>G) interrupting the 12-bp spacer region (shown in italics) is successfully amplified to produce the expected 19 CAG result. ( C , D ) PCR/CE genotyping of each SNP (rs473915 or rs76533208, respectively) results in the correct 19 CAG call. ( E ) A CAA frameshift (rs779781803) within the repeat sequence shows a repeat-primed amplification pattern and a terminal peak corresponding to 20 CAG, rather than 19 CAG, due to the mobility shift caused by the triplet interruption. Note that the CAA interruption causes a signal “dip” in the repeat-primed pattern; this dip flags result as suspicious for unexpected sequence variation.

Article Snippet: The AmplideX PCR/CE HTT Kit (cat#: 49657; Asuragen, Inc., Austin, TX, USA) was used to PCR amplify the HTT trinucleotide CAG fragment starting from 20 ng total of purified genomic DNA, isolated from peripheral blood leukocytes using the Gentra Puregene Blood Kit (Qiagen, Germantown, MD, USA), or the MagCore Genomic DNA Whole Blood Kit (Diatech Lab line, Jesi, Italy).

Techniques: Sequencing, Amplification, Mobility Shift

Journal:

Article Title: The Genus Aeromonas : Biochemical Characteristics, Atypical Reactions, and Phenotypic Identification Schemes

doi: 10.1128/JCM.41.6.2348-2357.2003

Figure Lengend Snippet: Type and reference cultures of Aeromonas strains used in this investigation a

Article Snippet: In this study we present cumulative biochemical data on almost 200 Aeromonas strains representing each of the 14 recognized species and propose possible schemes for their identification in the clinical laboratory (Table ). table ft1 table-wrap mode="anchored" t5 caption a7 Species No. of strains Type strain Reference culture(s) A. hydrophila 25 ATCC 7966 A. bestiarum 16 CDC 9533-76 (=ATCC 51108) ATCC 14715, NCMB 1134 A. salmonicida 13 CDC 434-84 (=Popoff C316) A. caviae 25 ATCC 15468 A. media 11 ATCC 33907 ATCC 35950, CDC 862-83 (=Popoff C239), CDC 435-84 (=Popoff C233) A. eucrenophila 9 NCMB 74 (=ATCC 23309) NCMB 73, LMG 16179, LMG 17062 A. sobria 2 CIP 7433 (=CDC 9538-76, =Popoff 208) CDC 9540-76 (=Popoff 215) A. veronii bv sobria b 25 ATCC 9071, CDC 437-84 (=ATCC 51106) A. jandaei 15 CDC 787-80 (=ATCC 49568) ATCC 49569, ATCC 49570, ATCC 49571 A. veronii bv veronii c 10 ATCC 35624 ATCC 35622, ATCC 35623, ATCC 35625, ATCC 35626 A. schubertii 12 CDC 2446-81 (=ATCC 43700) CDC 9180-81 (=ATCC 43701), CDC 2508-86 (=ATCC 43945), CDC 463-83 (=ATCC 43947) A. trota 16 AH2 (=ATCC 49657) MOB/A8 (=ATCC 49658), NMRI-208 (=ATCC 49661), AS-66 (=ATCC 49660), AS-370 (=ATCC 49659) A. encheleia 4 CECT 4342 (=ATCC 51929) CECT 4341 (=ATCC 51930), CECT 4340 (=ATCC 51931), CECT 4343 A. allosaccharophila 3 ATCC 51208 (=CECT 4199) ATCC 35942 (=CDC 715-84), LMG 14058 (=CECT 4200) A. popoffii 7 LMG 17541 (=CIP 105493) LMG 17542, LMG 17543, LMG 17544, LMG 17545, LMG 17546, LMG 17547 Open in a separate window a Abbreviations: ATCC, American Type Culture Collection (Manassas, Va.); CDC, Centers for Disease Control and Prevention (Atlanta, Ga.); NCMB, National Collection of Marine Bacteria (Aberdeen, Scotland); LMG, Laboratorium voor Microbiologie (Ghent, Belgium); CIP, Collection of the Institut Pasteur (Paris, France), CECT, Colección Española de Cultivos Tipo (Valencia, Spain). b Hybridization group 8, ornithine decarboxylase negative. c Hybridization group 10, ornithine decarboxylase positive.

Techniques: